| Title of the Research Problem |
Development of novel ECM-scaffold-based organoid models as a robust, high-throughput drug screening platform |
| Abstract of the Research problem |
Organoids are tiny, 3D structures that can mimic the functions of organs. Organoids mimic organ functions, but current methods of creating them are expensive and non-reproducible. To overcome this, we have developed a novel technology called ECM-ORGANOID, which is based on our patented ECM-mimetic glycosaminoglycan (GAG)-based scaffold. Unlike bio-sourced scaffolds, our technology is inexpensive, simple to follow, and highly reproducible. It can be used for drug screening with multiple readouts, such as biomarkers, cell viability, and metabolic response in the same construct. This will be useful for screening potential drug candidates targeting various disease pathways. |
| Target TRL to be achieved |
TRL-5 or above
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| Previous work done by the faculty |
The team has been working on the development of tumoroids to analyze drug activity. We have evaluated many different strategies for making reproducible tumoroids, including a unique technique for making cell-embedded organoids using glycosaminoglycan-based polymers. These organoids exhibited improved cell survival, proliferation, and function. Dr. Singh has previously developed a 3D-spheroid-based animal tumor model, which exhibited pathology akin to solid tumors observed in patients.
Our collaborator, Oncoseek Bio, has successfully developed a lung spheroid platform for screening antibodies and antibody-drug conjugates. They have also developed fibrosis spheroid for kidney cell lines (CKD), NAFLD/NASH spheroid, and several cancer spheroids.
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| Related Research Equipment / Lab Space / Facilities Available for solving this problem |
Cell culture facility, Advanced microscopy, Real-time PCR, Western blot, etc.
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| UN SDG Impacted |
UN-SDG-3 (Good Health and Well Being), UN-SDG-9 (Industry, Innovation, Infrastructure)
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| Ideal Applicant Background |
The ideal applicant can be from Biology or Pharmacy background. They should have significant hands-on experience in cell culture and molecular biology techniques.
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| Preferred Discipline of the Applicant |
Biology or Pharmacy
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| Faculty Name |
Aniruddha Roy
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| Faculty Web Page |
https://www.bits-pilani.ac.in/pilani/aniruddha-roy/
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